Composite

Part:BBa_M36224:Design

Designed by: Joel Dominguez, Ashwin Raghunathan, Denis Willett   Group: Stanford BIOE44 - S11   (2012-12-04)

Red Luciferase for E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal BglII site found at 240
    Illegal BglII site found at 1245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 680
    Illegal AgeI site found at 1211
    Illegal AgeI site found at 1337
    Illegal AgeI site found at 1658
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for use in E. coli. Repeats greater than 10bp removed and replaced.


Source

NCBI access number:AF139645

References

Kitayama, Y. et al. An in vivo dual-reporter system of cyanobacteria using two railroad-worm luciferases with different color emissions. Plant & cell physiology 45, 109–13 (2004)

Viviani, V. R., Bechara, E. J. & Ohmiya, Y. Cloning, sequence analysis, and expression of active Phrixothrix railroad-worms luciferases: relationship between bioluminescence spectra and primary structures. Biochemistry 38, 8271–9 (1999).